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How do you isolate Azospirillum?

How do you isolate Azospirillum?

Azospirilla are isolated from enrichment cultures by streaking on solid media, Congo Red agar (CRA) (Rodrı́guez Cáceres, 1982) being a particularly useful differential medium due to the scarlet coloration of Azospirillum colonies.

Which medium used for isolation of Azospirillum?

For the isolation of Azospirillum spp., Nfb semisolid medium was used. After 24 h incubation, the Nfb semi-solid medium showed white colored pellicle (Fig 1 & 2).

Is Azospirillum a fungi?

AM fungi are relatively nonspecific to host plant. For example, synergistic interactions between AM fungi along with a PGPR organism such as Azospirillum, Azotobacter, Bacillus, or Pseudomonas species, were found to be beneficial for enhancing plant growth and yield for a number of crops.

Who first isolated the Azospirillum organism?

Dr. Johanna Döbereiner
These short, rod-shaped, slightly curved Gram-negative bacteria were first isolated from soil in the Netherlands in 1925 [11]. Not many reports followed this first isolation until the group of Dr. Johanna Döbereiner ‘rediscovered’ Azospirillum in the mid-1970s.

How do you use azospirillum Biofertilizer?

Azospirillum is used for seedling root dip particularly for rice. 4 kg each of the recommended biofertilizers are mixed in 200 kg of compost and kept overnight. This mixture is incorporated in the soil at the time of sowing or planting. The inoculum should be applied 2-3 cm below the soil at the time of sowing.

How do you make azospirillum?

There are many small variations of preparing peat inoculant (depending on the desired formulation required) and most are small modification that are based on the following procedure: for preparation of 100 g of inoculant for Azospirillum spp., 45 g of ground peat (40 mesh) is thoroughly mixed with 5 g of CaCO 3 and 20 …

Is azospirillum symbiotic?

Azospirillum is a Gram-negative, microaerophilic, non-fermentative and nitrogen-fixing bacterial genus from the family of Rhodospirillaceae. Azospirillum bacteria can promote plant growth….

Scientific classification
Genus: Azospirillum Tarrand et al. 1979
Type species
Azospirillum lipoferum

What is use of Azospirillum?

Azospirillum can also promote plant growth by mechanisms of tolerance of abiotic stresses, named as induced systemic tolerance, mediated by antioxidants, osmotic adjustment, production of phytohormones, and defense strategies such as the expression of pathogenesis-related genes.

Can we spray Azospirillum?

Foliar Spray: ABTEC Azospirillum can be used for foliar spray. Mix 5-10 ml liquid in 1 litre water or 1 kg organic manure. For nursery application apply @ 500 ml/ 50 litres of water. Spray all over the plant or apply to the wet soil.

Where is Azospirillum found?

Azospirilla are mainly found in large numbers in tropical soils, but in various temperate zones, and even in tundra and semi-desert sites of the Canadian High Arctic [12] the isolation of Azospirillum spp. has been reported.

Is Rhizobium aerobic or anaerobic?

Complete answer: The Rhizobium is a bacteria which needs a symbiotic relationship to fix nitrogen. It is an aerobe, rod-shaped cell and gram-negative bacteria. It fixes nitrogen using nitrogenase enzymes. The nitrogenase easily gets oxidised in the atmosphere or during its aerobic conditions of surviving.

What is biofertilizer with example?

In strict sense, biofertilizers are not fertilizers. They are directly used for supply of nutrition to crop plants. Microorganisms such as bacteria, fungi, and blue green algae are largely used as biofertilizers. These organisms are added to the rhizosphere of the plant to enhance their activity in the soil.

How is the isolation of Azospirillum carried out?

The isolated strains were formed pellicles in NFb semisolid medium that is the characteristic feature of Azospirillum. Then transferred into NFb and BMS agar plats the colony was also observed. These isolated strains were confirmed by observation of gram negative staining.

Where can I find Azospirillum in paddy field?

In the present investigation, Azospirillum strain was isolated from the rhizosphere soil of paddy field. The nitrogen fixing bacteria Azospirillum was present in the dilution factor 10-5 , 10-6 , 10-7 . These isolated bacterial strains were confirmed by its morphological and physical characteristics on the growth on NFb and BMS medium.

What is the minimum detection limit for Azospirillum?

The minimum detection limit in real-time quantitative PCR analysis is 102 CFU g−1 in the seeded soil sample. Cells of the genus Azospirillum are Gram-stained negative, spiral or rod-shaped and non-spore-forming diazotrophic. Poly-β-hydroxybutyrate granules were observed after few days of incubation.

Which is the most representative species of Azospirillum?

This book summarizes basic and technical information related to the plant growth promoting rhizobacteria (PGPR) belonging to the genus Azospirillum, considered to be one of the most representative PGPR last 40 years.