What is a Tinctorial stain?
Tinctorial stains, commonly known as special stains, include a range of staining protocols involving dyes and chemical reactions which are employed upon tissue sections. The results are often colourful and yield useful diagnostic information for the Histopathologist. The undertaking of large-scale staining projects.
What does reticulin stain?
Reticulin Stain. The reticulin stain is extensively used in the histopathology laboratory for staining liver specimens, but can also be used to identify fibrosis in bone marrow core biopsy specimens.
What are the stains used in histopathology?
4.2 Important Histological Stains Used in the Past and Present
- Carmine.
- Hematin and Hematoxylin.
- Silver Nitrate.
- The Hematoxylin and Eosin Procedures.
- Romanowsky Stains–Giemsa Stains.
- Gram Stain.
- Trichrome Stains.
Which stain is used in the demonstration of carbohydrates?
Periodic Acid Schiff
Periodic Acid Schiff (PAS) Staining: A Useful Technique for Demonstration of Carbohydrates.
What is meant by Tinctorial?
: of or relating to colors or to dyeing or staining also : imparting color.
What are histochemical stains?
A staining method used to detect polysaccharides such as glycogen, and mucosubstances such as glycoproteins, glycolipids, and mucins in tissues and fungal hyphae.
Why are special stains used?
“Special stains” are processes that generally employ a dye or chemical that has an affinity for the particular tissue component that is to be demonstrated. They allow the presence/or absence of certain cell types, structures and/or microorganisms to be viewed microscopically.
What stain is helpful in diagnosing Wilson’s disease?
Wilson disease is often fatal if not recognized and treated when symptomatic. Wilson disease biopsy specimen with rhodanine stain (stain specific for copper deposition).
What are the three types of stains?
Stains can be classified into the following types, depending upon its chemical nature and the type of staining methods. Based on chemical nature: There are three kinds of stain, acidic, basic and neutral, depending upon the chemical nature of the stain. Based on the staining method: There are four kinds of stain, viz.
What two stains are used in an H&E stain?
As its name suggests, H&E stain makes use of a combination of two dyes – haematoxylin and eosin. Tissue stained with haematoxylin and eosin shows cytoplasm stained pink-orange and nuclei stained darkly, either blue or purple. Eosin also stains red blood cells intensely red.
What other specific molecule found in the brain can Alcian blue stain for?
PURPOSE: Alcian blue stains acid mucosubstances and acetic mucins.
Which fixative is used for histochemical techniques?
Acetone. Acetone (CH3COCH3) has a similar action to alcohol and has been used as a fixative and dehydrant for tissue processing, particularly rapid hand-processing of small specimens. It is widely recommended for fixation as part of the histochemical demonstration of enzymes where it is generally used cold (4°C).
When do you stain for endospores and capsules?
This technique is also used when you stain for endospores and capsules. Just as in preparing a smear, you only need a small amount of organism. If you have too many organisms, you won’t be able to see the morphology of individual cells. It is also important not use too much nigrosin.
Which is the best stain for Corynebacterium diphtheriae?
It is also the stain of choice for identifying the metachromatic granules in Corynebacterium diphtheriae. The granules will stain a distinctly deeper blue than the surrounding blue bacteria. Other species of Corynebacterium do not have the metachromatic granules.
What does special staining mean in histopathology?
Eosin Y is most popular. The term special stains traditionally referred to any staining other than an H&E. It covers a wide variety of methods that may be used to visualize particular tissue structures, elements, or even microorganisms not identified by H&E staining.
Which is the best stain for oral infections?
Methylene blue is used to determine the morphology of fusiform and spirochetes in oral infections. It is also the stain of choice for identifying the metachromatic granules in Corynebacterium diphtheriae.