What is mCherry antibody?
mCherry is a fluorescent protein derived from Discosoma sp. Mushroom corals. Our antibodies for mCherry can be used to detect native and denatured forms of mCherry or mCherry fusion proteins in various applications.
Do GFP antibodies recognize mCherry?
A: The anti-GFP antibody was made using the N-terminal of GFP as an antigen and will not react with RFP proteins dsRED or mCherry, since their sequences are so different.
Does mCherry antibody recognize tdTomato?
The mCherry Monoclonal Antibody differentiates red fluorescent proteins. The mCherry Monoclonal Antibody recognizes mCherry but not tdTomato, DsRed-Express2, or DsRed-Monomer.
What stains mCherry?
mCherry-Rabbit mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in transfection and transgenic experiments. As shown below, the antibody does not stain any protein band in untransfected HEK293 cells.
What is the molecular weight of EGFP?
32.7 kDa
Product Details
| Alternate Name | E-GFP, Enhanced Green Fluorescent Protein |
|---|---|
| Appearance | Lyophilized protein |
| Physical Form Description | Freeze Dried |
| Molecular Weight | 32.7 kDa |
| Purity by SDS-PAGE | ≥97% |
Does GFP antibody recognize EGFP?
GFP antibody (ab6556) is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP, RFP and EGFP.
How do you find an RFP?
RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm.
Does mCherry antibody recognize RFP?
The anti-RFP antibody 5F8 recognizes common RFP variants including mCherry, mPlum, mRFP, mRFPruby.
Does GFP antibody recognize RFP?
The antibodies are also confirmed for non-reactivity to GFP protein. In addition, conjugation of RFP antibodies to either fluorescent dyes, biotin or horseradish peroxidase are available to further facilitate RFP protein detection and quantification.
How do you detect mCherry?
It’ll depend on the flow cytometer you have access to but to best detect the mCherry signal excite with the yellow-green laser at 561 nm and detect in the PE-TexasRed channel with a 610/20 bandpass filter.
What does mCherry stain for?
Which is the best Antibody to test for mCherry?
Immunofluorescent analysis of HEK293 cells transfected with pFin- EF1-mCherry vector labeling mCherry with ab167453 at 1/500 (red). Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Blue DNA stain. Publishing research using ab167453?
Where does the mCherry protein in GFP come from?
The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma. Immunofluorescent analysis of HEK293 cells transfected with pFin- EF1-mCherry vector labeling mCherry with ab167453 at 1/500 (red).
How is the mCherry Mab used in cell signaling?
Confocal immunofluorescent analysis of A549 cells transiently transfected with an mCherry construct, using mCherry (E5D8F) Rabbit mAb and Anti-rabbit IgG (H+L), F (ab’)2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 (green). Colocalization of green and red signals appear yellow in the composite image (bottom-right).
Can a mCherry antibody cross react with GFP?
This mCherry Antibody (1C51) does not cross react with GFP. Test in a species/application not listed above to receive a full credit towards a future purchase. Use in Flow reported in scientific literature (PMID:33335127). Use in IHC and IHC-P reported in scientific literature (PMID: 27396338 and 27716840 respectively).